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EXTRACELLULAR VESICLES IN DIAGNOSTICS AND PRECISION MEDICINE
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EXTRACELLULAR VESICLES IN DIAGNOSTICS AND PRECISION MEDICINE
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Academic year 2024/2025
- Course ID
- BIO0244
- Teacher
- Benedetta Bussolati
- Year
- 1st year
- Teaching period
- Second semester
- Type
- Elective
- Credits/Recognition
- 4
- Course disciplinary sector (SSD)
- MED/46 - medical and biotechnology laboratory techniques
- Delivery
- Formal authority
- Language
- English
- Attendance
- Obligatory
- Type of examination
- Oral
- Type of learning unit
- corso
- Prerequisites
- In order to understand the contents of this module, the basic knowledge on cell biology and physiology is needed for the understanding of processes involved in extracellular vesicle generation and activity.
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Sommario del corso
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Course objectives
Extracellualr vesicles are cell-released vesicles present in body fluids, and their cargo reflects the pathophysiological state of the cells. The course will present the current knowledge on EV charaterization and use for diagnostic and therapeutic purposes, and the main technical approaches. Moreover, it will provide knowledge on vesicle engineering to modify cargo and targeting properties. The goal is to give the students the basic knowledge to start working on projects involving the isolation and characterization of nanovesicles. This topic is of increasing interest for diagnostic and therapeutic applications in academia, pharmaceutical and industry sectors.
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Results of learning outcomes
The student will gain knowledge and technical skills on extracellular vesicle in diagnostic and in therapeutic application, with a focus on regenerative medicine.
Results of learning outcomes
The student will gain knowledge and technical skills on extracellular vesicle in diagnostic and in therapeutic application, with a focus on regenerative medicine. In particular, students will be able to:
- Understand the biological processes involved in vesicle generation, release and uptake.
- Learn the main techniques that can be used for pre-clinical studies involving vesicles.
- Apply the main isolation and characterization techniques.
- Critically evaluate papers describing the use of engineered vesicles in pre-clinical studies.
- Prepare and explain a presentation on one or more scientific papers involving the use of vesicles for regenerative medicine.
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Program
Extracellular vesicle biogenesis
Vesicle characterization. tools and analyses
Platforms for EV diagnostics and possible applications
EV engineering and loading
EV surface functionalization and biodistribution
EV-mimetics
Vesicles in regenerative medicine
Technical lesson on vesicle isolation and characterization
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Course delivery
The course will be delivered through classical frontal lessons and power point presentations (20 h), coupled to seminars and article discussion held by the students (4 h), and technical lessons (8 h).
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Learning assessment methods
The final exam will be a power point project presentation on one of the program topics, chosen by the student, with a discussion on the different aspects of the course. It will be evaluated the student’s acquisition of:
- Scientific terminology
- Ability to present summarized scientific data
- Interpretation of scientific data
- Application of the acquired knowledge on extracellular vesicles to a scientific problem regarding regenerative medicine
Suggested readings and bibliography
- Enroll
- Open
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