- Oggetto:
Fundamentals of molecular biology
- Oggetto:
Fundamentals of molecular biology
- Oggetto:
Academic year 2024/2025
- Course ID
- BIO0260A
- Teacher
- Isaia Barbieri (Lecturer)
- Year
- 1st year
- Teaching period
- First semester
- Type
- Distinctive
- Credits/Recognition
- 3
- Course disciplinary sector (SSD)
- BIO/11 - molecular biology
- Delivery
- Formal authority
- Language
- English
- Attendance
- Obligatory
- Type of examination
- Quiz
- Type of learning unit
- modulo
- Modular course
- CELL AND MOLECULAR BIOLOGY IN DIAGNOSTICS (BIO0260)
- Propedeutic for
- APPLIED CELL AND MOLECULAR BIOLOGY IN DIAGNOSTICS BIO0261
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Sommario del corso
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Course objectives
The principles of molecular biology module is aimed at providing a synthetic view of the basic molecular biology knowledge normally acquired in the undergraduate course of biology and biotechnology, tailored to provide all the bases to follow the more applied second part. The objectives of this module consist in providing solid information about the biochemical structure of DNA and RNA, DNA dynamic organization within the chromatin, the replication and recombination mechanisms, DNA repair, and transcriptional and epigenetic regulation of gene expression, including the role of non-coding RNAs in regulating gene expression.
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Results of learning outcomes
At the end of the course, the student should have a deep knowledge of:
- biochemical structure of DNA and RNA.
- DNA dynamic organization within the chromatin
- replication and recombination mechanisms
- DNA repair mechanisms
- DNA recombination
- transcriptional and epigenetic regulation of gene expression,
role of non-coding RNA in regulating gene expression.
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Program
DNA
- Intro centrality of DNA: Avery experiments and central dogma of Molecular Biology
- DNA structure
- RNA structure
- Chromatin structure, its remodeling and DNA methylation.
- Nucleosomes
- Histones, reversible post-translational modifications
- Histone code, i.e. the biochemical implications of Histone modifications
- DNA methylation and its biochemical implications
- Molecular biology laboratory techniques
Chromosomes:
- Telomeres, centromeres
- Chromosomes duplication:
- Chromatin replication
- DNA replication
Genome Expression
- Transcription
- Transcription regulation (cooperativity, repression, ..)
- epigenetics, nucleosome positioning and remodelling, readers,
- gene expression factory: elongation, maturation, splicing, transport, non-sense mediated decay
- DNA topology, Insulator
- mRNA functions
- mRNA structure
- Ribosomal RNA e tRNA
- lincRNA –
- small structural RNAs (snoRNA, miRNA)
- mRNA translation and its regulation
DNA repair
- DNA damage and chemical modifications
- DNA repair mechanisms
- Biochemical and biological consequence of defective DNA repair
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Course delivery
START UNITO module consisting of pre-recorded lectures and knowledge assessment questionnaires available online.
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Learning assessment methods
Learning assessment related to the Start module will be assayed by a multiple choice test after the completion of the Module and questionnaires online.
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Support activities
Further material such as scientific papers and other material available online will be suggested to deepen discussed topics.
Suggested readings and bibliography
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- Book
- Title:
- Lewin's Genes XII
- Year of publication:
- 2018
- Publisher:
- Jones & Bartlett Learning
- Author:
- JOCELYN E. KREBS, ELLIOTT S. GOLDSTEIN, STEPHEN T. KILPATRICK
- ISBN
- Permalink:
- Required:
- Yes
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- Book
- Title:
- Biologia molecolare del gene
- Year of publication:
- 2022
- Publisher:
- Zanichelli
- Author:
- Watson - Baker - Bell - Gann - Levine - Losick
- ISBN
- Required:
- Yes
- Enroll
- Open
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